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Mass 4.68 mg validated accurate dosing, confirming the reliability of LeoLabs production process

A 0.5 mL (50 unit) insulin syringe has finer gradation marks, making doses below 25 units easier to measure

These sequences were then run through an R script (Supplementary Materials, bash_DADA2 + IDTAXA_workflow_Illumina-PairedEnd_V1V3.R) which trimmed reverse reads to 260 bp, removed forward reads exceeding a maximum expected error of three, and truncated reverse reads exceeding a maximum expected error of six

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