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Example Integrated Workflow Conduct UVVis titration to locate binding saturation

The brain RAS, particularly AngIV, has been implicated in learning, memory, neuroprotection, and cerebral blood flow regulation

During this process, the target protein is attached to a solid-phase carrier (e.g., avidin-streptavidin tags, GST tags, His tags), where it interacts with other proteins in the solution

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Practice using numbing cream if required, and thoroughly smooth the injection site