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cDNA was diluted tenfold with water and quantitative PCR assays were performed in a StepOnePlus real-time PCR system (Applied Biosystems, Waltham, MA) using TaqMan chemistry
Membranes were probed with various primary antibodies overnight at 4 C
More human studies are needed to know if green tea is helpful in reducing IGF-1 and, if so, how much is needed per day
2.4 mg)
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Journal of Experimental Orthopaedics, 2016
Mitochondrial Energy Production Mitochondria produce ATP, the bodys main source of cellular energy
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